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  5. Cytiva TempliPhi™ 100/500 Amplification Kit

Cytiva TempliPhi™ 100/500 Amplification Kit

Catalog No. 45002262
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No. of Reactions:
100
500
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Catalog No. No. of Reactions
45002262 100
45002265 500
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Catalog No. 45002262 Supplier Cytiva Supplier No. 25640010
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Description

Includes

Sample buffer, Reaction buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/μL)

Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.

  • Generate microgram quantities of template DNA from picogram amounts of starting material
  • Completed reactions contain uniform quantities of DNA irrespective of input amount, allowing for use directly in sequencing reactions without quantification
  • Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA without the need for purification
  • Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation
  • Contain separate, unmixed components for enhanced stability

Specifications

Content And Storage The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification
Description illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
For Use With (Application) Sequencing Template Amplification Kits and Reagents
Includes 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA
No. of Reactions 100
Product Type illustra TempliPhi 100 Amplification Kit
Test Time 4-18 h depending on starting material