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PeproTech IL-2 Polyclonal Antibody, PeproTech®, Invitrogen™

Numéro de catalogue. 500P274100
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50 μg
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Numéro de catalogue. Quantity
500P274100 100 μg
500P27450UG 50 μg
500P2741MG 1 mg
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Numéro de catalogue. 500P274100 Fournisseur PeproTech Code fournisseur 500P274100UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAIAQSI ISTM. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-2. Anti-Rat IL-2-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-2 matrix. Sandwich ELISA: To detect Rat IL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-2 (500-P274Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-2. Western Blot: To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Spécifications

Antigen IL-2
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene IL2
Gene Accession No. P17108
Gene Alias aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Symbols IL2
Host Species Rabbit
Immunogen E.coli-derived Recombinant Rat IL-2.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 116562
Target Species Rat
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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