Invitrogen™ iBind PLUS Solution Kit

• The iBind PLUS Solution comes ready to use with no preparation required. It is used to prewet the iBind card prior to the run and acts as the block and wash buffer for the membrane processing, helping wash away unbound primary and secondary antibodies.
• The iBind PLUS Antibody Diluent is used to dilute the primary and secondary antibodies. In most experiments, the primary antibody can be diluted to the same recommended concentration as used for traditional blotting, however only 2 mL of prepared primary antibody is required with iBind, saving up to 80% of primary antibody consumption. The iBind PLUS Antibody Diluent is specially formulated with bulking agents and stabilizing molecules to improve antibody binding with the target antigen to increase signal intensity.

During a run on the iBind, each solution is released from the iBind device wells through sequential lateral flow (SLF) and are wicked over the iBind card towards the stack region, incubating the membrane with each reagent. The glass fiber matrix of the iBind card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

The iBind PLUS Solution Kit, together with the iBind Western Device (Cat. No. SLF1000) and iBind Cards (Cat. No. SLF1010), bring automation and simplicity to western blotting with efficient and hands-free immunoprocessing in as little as 3 hours. Compared to manual methods, the iBind western system requires less primary antibody, enables reproducible results, and is compatible with downstream detection protocols.

Each iBind PLUS Solution Kit contains sufficient reagents for 10 mini-size blot immunoprocessing runs.

For iBind Flex, use the iBind Flex PLUS Solution Kit. For fluorescent applications, use the iBind Fluorescent Detection (FD) Solution Kit (Cat. No. SLF1019) or iBind Flex Fluorescent Detection (FD) Solution Kit (Cat. No. SLF2019).