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Invitrogen™ Silencer™ Cy™3-labeled Negative Control No. 1 siRNA

Catalog No. AM4621
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AM4621 5 nmol
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Catalog No. AM4621 Supplier Invitrogen™ Supplier No. AM4621
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Description

Silencer™ Cy™3-labeled Negative Control No. 1 siRNA

Invitrogen™ A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

  • Simplifies the monitoring of siRNA delivery
  • Functionally tested in several common cell lines
  • HPLC purified, duplexed, and ready to use

    Visualize siRNA Uptake with Labeled siRNAs
    Fluorescent labeled siRNAs allow for the direct observation siRNA cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours. Fluorescent labeling of siRNAs allows for rapid monitoring of siRNA delivery by fluorescence microscopy, as opposed to unlabeled siRNAs, whose delivery must be monitored by target gene knockdown. The Silencer™ Cy™3 Labeled Negative Control #1 siRNA sequence is identical to that of the Invitrogen Silencer™ Negative Control# 1 siRNA (SKU #AM4611), but with the fluorescein derivative Cy™3 (excitation max. = 547, emission max. = 563 nm), on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light. Silencer™ Cy™3 Labeled Negative Control #1 siRNA is suitable for monitoring siRNA uptake by chemical transfection or electroporation. Cy™3 labeling is recommended over FAM™ labeling when using electroporation because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection.

    Accessory Products:
    Silencer™ FAM™ Labeled Negative Control #1 siRNA (SKU #AM4620) is also available. Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Invitrogen™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

    • Specifications

    Content And Storage The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.
    Control Type Negative Control
    RNAi Type siRNA
    For Use With (Application) Transfection, RNAi
    Label or Dye Cy3
    Product Line Silencer, Ambion
    Product Type siRNA
    Purity HPLC
    Quantity 5 nmol
    Shipping Condition Dry Ice

    Frequently Asked Questions (FAQs)

    How critical is it to include positive and negative controls in siRNA experiments?

    A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.
    What are the positive and negative controls I should use with my Silencer or Silencer Select siRNA experiment?

    We offer several negative and positive controls for both Silencer and Silencer Select siRNA.

    For Silencer siRNA positive control:
    - Silencer Firefly Luciferase (GL2 + GL3) siRNA (Cat. No. AM4629, 5nmol)
    - Silencer GAPDH siRNA (human) (Cat. No AM4605, 5nmol; AM4633, 40 nmol)
    - Silencer GAPDH siRNA (human, mouse, rat) (Cat. No. AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
    - Silencer GFP (eGFP) siRNA (Cat. No. AM4626, 5 nmol)
    - Silencer KIF11 (Eg5) siRNA (human, mouse, rat) (Cat. No. AM4639, 5 nmol)

    If working with Fluorescently labeled Silencer siRNA:
    - Silencer Cy-3 labeled GAPDH siRNA (human, mouse, rat) (Cat. No. AM4649, 5 nmol)
    - Silencer FAM-labeled GAPDH siRNA (human, mouse, rat) (Cat. NO. AM4650, 5 nmol)

    If working with Silencer siRNA in vivo ready:
    - Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

    For Silencer siRNA negative controls:
    - Silencer Negative Control No. 1 siRNA (Cat. Nos. AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
    - Silencer Negative Control No. 2 siRNA (Cat. Nos. AM4613, 5 nmol; AM4637, 30 nmol)
    - Silencer Negative Control No. 3 siRNA (Cat. No. AM4615, 5 nmol)
    - Silencer Negative Control No. 4 siRNA (Cat. No. AM4641, 5 nmol)
    - Silencer Negative Control No. 5 siRNA (Cat. No. AM4642, 5 nmol)

    If working with Fluorescently labeled Silencer siRNA:
    - Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No AM4621, 5 nmol)
    - Silencer FAM-labeled Negative Control No. 1 siRNA (Cat. No. AM4620, 5 nmol)

    If working with Silencer siRNA in vivo ready:
    - Silencer in vivo ready (Cat. No. 4404021)

    For Silencer Select siRNA Positive control:
    - Silencer Select GAPDH Positive Control siRNA (Cat. No. 4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
    - Silencer Select MALAT1 Positive Control siRNA for non-coding siRNA (Cat. No. 4455877, 5 nmol)

    For Silencer Select siRNA negative control:
    - Silencer Select Negative Control No. 1 siRNA (Cat. No. 4390843, 5 nmol; 4390844, 40 nmol)
    - Silencer Select Negative Control No. 2 siRNA (Cat. No. 4390846, 5 nmol; 4390847, 40 nmol)

    If working with Silencer Select siRNA in vivo ready:
    - Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250nmol)

    What is the resuspension protocol for Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No. AM4621)?

    We recommend resuspending Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No. AM4621) in nuclease-free water at a stock concentration of 50 µM by adding 100 µL of water to the 5 nmol siRNA. Resuspended siRNA can be stored at -20 degrees C.

    For Research Use Only. Not for use in diagnostic procedures.