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Invitrogen™ BacLight™ Red Bacterial Stain
Description
The BacLight Green (Cat. no. B-35000) and BacLight Red (Cat. no. B-35001) bacterial stains are fluorescent labeling reagents for detecting and monitoring bacteria. These two dyes are not nucleic acid stains. Bacteria stained with the BacLight Green and BacLight Red bacterial stains exhibit bright green and red fluorescence (absorption/emission ∼480/516 and ∼581/644 nm, respectively), and can be resolved using the appropriate flow cytometric channels or fluorescence microscopy filters. The BacLight bacterial stains are compatible with formaldehyde or alcohol fixation methods.
Specifications
Specifications
| Cell Type | Bacteria |
| Content And Storage | Contains 20 vials of BacLight™ red bacterial stain (50 ug per vial). Store in freezer (-5 to -30°C) and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
| Product Type | Bacterial Stain |
| Dye Type | Other Label(s) or Dye(s) |
| Emission | 488 |
| Form | Solid |
| Format | Tube(s), Slide(s) |
| Product Line | BacLight |
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Frequently Asked Questions (FAQs)
What bacterial parameters can I look at by flow cytometry?
You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).
For Research Use Only. Not for use in diagnostic procedures.