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ProSci Human Skin Lysate
Description
- Skin tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors)
- Tissue and cell debris was removed by centrifugation
- The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Spécifications
Spécifications
| Tissu | Skin |
| Quantité | 0.1 mg |
| Condition de tissus | Normal |
| Espèces hôtes | Human |
| Concentration | 2 mg/mL |
| État de la protéine | Denatured |