Recombinant Protein;100UG;Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (OXL-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1).;This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.3 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human OX40, Fc Tag (Cat. No. OX0-H5255) at 0.5 g/mL (100 L/well) can bind Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. OXL-H5250) with a linear range of 0.8-13 ng/mL (QC tested).;FACS assay shows that recombinant Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. OXL-H5250) can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand is 0.003 g/ml (Routinely tested).;FACS analysis shows that the binding of Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. OXL-H5250) to 293T overexpressing OX40 was inhibited by increasing
