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ProSci Human Heart Lysate

Catalog No. 16916783
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0.1 mg
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Catalog No. Quantity
16916783 0.1 mg
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Catalog No. 16916783 Supplier ProSci Supplier No. 13010100
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Description

Total protein normal heart - Human

  • Heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin).
  • Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
  • The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Specifications

For Use With (Application) Western Blot
Tissue Heart
Quantity 0.1 mg
Storage Buffer Heart lysate is supplied in SDS sample buffer containing 50 mM DTT.
Tissue Condition Normal
Host Species Human
Concentration 2 mg/mL
Product Type Lysate
Content And Storage Store at 2°C to 8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Protein State Denatured