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Gibco™ Human Epidermal Keratinocytes, Pooled (HEKp)

Catalog No. A13401
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Description

Human Epidermal Keratinocytes, pooled (HEKp)

Human Epidermal Keratinocytes, pooled (HEKp) from Invitrogen are normal cells isolated from multiple neonatal foreskins. These cells are optimal for investigating the molecular and biochemical bases of normal and disease processes of the skin. They have been cryopreserved at the end of the primary culture stage in animal origin-free media containing 10% DMSO. Use HEKp for your research into fibrosis, wound healing, irritancy, and diseases such as psoriasis.
  • Guaranteed to have ≥70% viability upon thawing
  • Each vial guaranteed to reach ≥15 population doublings after thaw
  • Sufficient cells to seed ≥100 cm2
  • Tested for potential biological contaminants
  • Optimized for use in EpiLife™ serum-free media

    Get More Physiologically Relevant Results with Invitrogen Primary Cells
    Human primary cell cultures closely mimic the in vivo state and thus generate more physiologically relevant data. Our Human Epidermal Keratinocytes are isolated and pooled from 4-6 neonatal foreskins. This process is robust and yields large donor lots to ensure availability of consistent product for your studies.

    Reliable Performance and Purity
    Primary cells from Invitrogen are guaranteed to have ≥70% viability upon thawing with each vial containing sufficient cells to seed ≥100 cm2 of surface area. Performance testing guarantees each vial of HEKp will reach ≥15 population doublings after thaw. In addition, each lot is tested for potential biological contaminants--HIV-1, Hepatitis B, Hepatitis C viruses, mycoplasma, bacteria, yeast, and other fungi-- to ensure quality and robustness.

    Optimized for Use with Gibco™ Media
    Human Epidermal Keratinocytes, pooled, from Invitrogen have been optimized for use with our serum-free media, specifically EpiLife™ Medium with 60μM calcium and Human Keratinocyte Growth Supplement (HKGS). Preparation of supplemented media is a simple process; the final solution can be stored for up to 30 days at 4°C.

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

    Learn more about our complete system of media and supplements for culturing Human Epidermal Keratinocytes

    • Specifications

    Cell Passage Number Primary culture stage
    Classification Serum-Free
    Content And Storage ≥ 1 × 106 viable cells
    Age Newborn (14 days or less)
    Cell Type Keratinocyte Cells
    Culture Type Adherent Cell Culture
    Form Cryopreserved
    Gender Male
    Species Human
    Donor Attributes Normal
    Donor Source Pooled
    Format Vial
    No. of Cells ≥1 x 106
    Product Line Gibco
    Product Type Human Epidermal Keratinocyte
    Quantity 1 vial
    Tested For Hepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1
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    Frequently Asked Questions (FAQs)

    I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

    These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

    I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

    These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

    What is the doubling time for Human Keratinodyte?

    Each lot is tested for doubling time and the results are listed on the COA.
    Can I specify a donor type for the primary cells you offer?

    This information also appears on the COA.
    What is the difference between basal cells or basal keratinocytes?

    These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.
    What media do you use during the first passage of primary cells?

    We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
    How is the population doubling calculated for primary cells?

    Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

    What are typical primary cell yields from tissue?

    It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

    Are primary cells isolated from tissue using enzymatic digestion?

    Yes, our primary cells are isolated from tissue using enzymatic digestion.

    What are primary cells?

    Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. These cells have undergone very few population doublings and are therefore more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines, making primary cells a more representative model for the in vivo state.

    Primary cells from different species may be used, allowing you to highlight potential differences between humans and preclinical test species. Before in vivo studies, mouse or rat cells can be used to refine doses and reduce the number of animals required for preclinical toxicology. Human cells can be used to determine the accuracy of extrapolating human data from an animal model.

    For Research Use Only. Not for use in diagnostic procedures.