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ProSci Human Brain Lysate

Catalog No. 16916796
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Quantity:
0.1 mg
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Catalog No. Quantity
16916796 0.1 mg
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Catalog No. 16916796 Supplier ProSci Supplier No. 13030100
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Description

Total protein normal brain - Human

  • Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
  • Tissue and cell debris was removed by centrifugation
  • Protein concentration was determined with Bio-Rad protein assay
  • The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Specifications

Tissue Brain
Quantity 0.1 mg
Tissue Condition Normal
Host Species Human
Concentration 2 mg/mL
Protein State Denatured