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Cytiva HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns

Catalog No. 45002533
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Product Type:
HiScreen Capto DEAE IEX Column
HiScreen Capto Q IEX column
HiScreen Capto S IEX column
Ligand Type:
Diethylaminoethyl
Quaternary Amine
Sulphonate group
pH Range:
2 to 12
2 to 9
4 to 12
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Catalog No. Product Type Ligand Type pH Range
45002533 HiScreen Capto DEAE IEX Column Diethylaminoethyl 2 to 9
45002529 HiScreen Capto Q IEX column Quaternary Amine 2 to 12
45002530 HiScreen Capto S IEX column Sulphonate group 4 to 12
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Catalog No. 45002533 Supplier Cytiva Supplier No. 28926982

Description

Designed for method optimization and parameter screening

  • Fast, efficient, and cost-effective capture and intermediate protein purification
  • Ready-to-use columns for convenient process development optimization
  • Highly cross-linked agarose with dextran surface extender matrix
  • Bed height of 10cm is designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

Capto S: Strong cation (sulfonate group) exchange BioProcess™ medium
Capto Q: Strong anion (quartenary amine group) exchange BioProcess medium
Capto DEAE: Weak anion (diethylaminoethyl group) exchange BioProcess medium

Specifications

Buffer 1M Acetic Acid, 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Product Type HiScreen Capto DEAE IEX Column
Bed Volume 4.7mL
Diameter (English) Inner 0.3 in.
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Height (English) 3.93 in.
Height (Metric) 100mm
Flow Rate <700 cm/hr.
Material Polypropylene
Ligand Type Diethylaminoethyl
Max. Pressure 3 bar
pH Range 2 to 9
Particle Size 45 to 165μm
Support Type Highly cross-linked Agarose with Dextran surface extender
Content And Storage 4° to 30°C
Column Type Ion Exchange Column
For Use With (Application) Modern large-scale manufacturers for fast, efficient and cost-effective capture and intermediate Protein purification, optimization and parameter screening
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