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Invitrogen™ HCS CellMask™ Stains

Catalog No. H32720
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Description:
HCS CellMask Blue Stain
HCS CellMask Deep Red Stain
HCS CellMask Green Stain
HCS CellMask Near-IR Stain
HCS CellMask Orange Stain
HCS CellMask™ Red Stain
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Catalog No. Description
H32720 HCS CellMask Blue Stain
H32714 HCS CellMask Green Stain
H32722 HCS CellMask Near-IR Stain
H32713 HCS CellMask Orange Stain
H32712 HCS CellMask™ Red Stain
H32721 HCS CellMask Deep Red Stain
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Catalog No. H32720 Supplier Invitrogen™ Supplier No. H32720
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Description

Easily visualize entire cells or individual cell structures during high-content screening (HCS) assays with HCS CellMask stains, which are available in a variety of colors for multiplexing flexibility and can be used immediately after fixation and permeabilization, or after antibody labeling.

Identify entire cells (cytoplasm and nucleus) during high-content screening (HCS) assays with the versatile HCS CellMask Red, CellMask Orange, CellMask Green, CellMask Blue, CellMask Deep Red, and CellMask Near-IR stains, which are fluorophores that can be used immediately after fixation and permeabilization, or after antibody labeling. They are available in a variety of colors for multiplexing protocols.

The versatile HCS CellMask Red, CellMask Orange, CellMask Green, CellMask Blue, CellMask Deep Red, and CellMask Near-IR stains are cell delineation tools for high content screening (HCS) platforms, label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. The HCS CellMask Red and Blue stains replace the HCS CellMask Red cytoplasmic/nuclear (Cat. No. H32711) and HCS CellMask Blue cytoplasmic/nuclear stains (Cat. No. H34558), respectively.

HCS CellMask stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask stains can be used for measuring DNA content in addition to enabling robust cell demarcation. HCS NuclearMask stains are also available in a choice of colors for multiplexing flexibility.

Order Info

Shipping Condition: Room temperature

Specifications

Color Blue
Content And Storage Includes 1 set of vials, providing sufficient material to stain a total of ten 96-well microplates.

Store at ≤-20°C, desiccate, and protect from light.
Excitation Wavelength Range 346/442
For Use With (Equipment) High Content Instrument
Quantity 1 Set
Detection Method Fluorescence
Description HCS CellMask Blue Stain
Emission Visible
Product Line CellMask
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Stain
SubCellular Localization Cytoplasm, Cytosol, Nucleus
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Frequently Asked Questions (FAQs)

I need a total cell stain, similar to HCS CellMask Blue stain, to label cytoplasm and nuclei in live cells. What do you recommend?

You can use a combination of two dyes with overlapping blue emission. For cytoplasm, you can label the cell with CellTracker Blue CMAC. This can be combined with Hoechst 33342 for nuclei. Both dyes are imaged using a standard DAPI filter set.
What dyes are used to make the CellMask stains?

The proprietary fluorescent dyes in the CellMask stains are general cytoplasmic stains. They are not found to bind to any specific cellular component.