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Gibco™ HBSS (10X), no calcium, no magnesium, phenol red
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Catalog No. 14180046
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14180046 500 mL
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Catalog No. 14180046 Supplier Gibco™ Supplier No. 14180046
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Description

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This HBSS is modified as follows:

  • With: Glucose, Phenol Red
  • Without: Calcium, Magnesium, Sodium Bicarbonate

The complete formulation is available.

Concentrated forms of Gibco™ HBSS require pH adjustment and filtration at the time of preparation (see protocol for details).

Specifications

Chemical Name or Material Hanks' Balanced Salt Solution (HBSS)
Concentration 10X
pH 5.6 to 6.1
Physical Form Liquid
Product Line Gibco
Quantity 500 mL
Solution Type Hank's Balanced Salt
Sterility Sterile-filtered
Includes No Calcium, No Magnesium
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Osmolality 2600–2900 mOsm/kg
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Frequently Asked Questions (FAQs)

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.
Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).
Is it true that phenol red has estrogenic properties?

Phenol red in tissue culture is a weak estrogen. Implications concerning study of estrogen-responsive cells in culture are discussed in Proc Natl Acad Sci U S A 83:2496 (1986).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.