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Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Description
The all-in-one Thermo Scientific™ Halt Protease and Phosphatase Inhibitor Cocktail provides the convenience of a single solution with full sample protection.
This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale.
Highlights:
- Complete protection – all-in-one cocktail contains both protease and phosphatase inhibitors
- Multiple package sizes – choose from four convenient packages sizes to match nearly any scale
- Convenient – the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
- Easy storage – aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
- Compatible – can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
- Validated – fully compatible with Thermo Scientific Pierce™ Cell Lysis Buffers
Includes:
Formulations with and without supplementary vial of EDTA for metalloprotease inhibition, both of which are offered in four package sizes.
Storage Requirements:
Upon receipt store at 4°C. Do not freeze product.
Specifications
Specifications
| Description | Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free |
| Product Type | Protease and Phosphatase Inhibitor Cocktail |
| Content And Storage | Upon receipt store at 4°C. Product shipped with an ice pack. Do not freeze product. |
| Form | Liquid |
| Product Line | Halt |
| Buffer | Lysis Buffer |
| Reagent Type | Protease Inhibitor, Phosphatase Inhibitor |
| Inhibitors | Protease Inhibitor, Phosphatase Inhibitor |
| Quantity | 10 mL |
Frequently Asked Questions (FAQs)
The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.
Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.
Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.
EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.
The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).
For Research Use Only. Not for use in diagnostic procedures.