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  6. Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Catalog No. PI78442
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PI78442 24 x 100 μL
PI78446 10 mL
PI78440 1 mL
PI78444 5 x 1 mL
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Catalog No. PI78442 Supplier Thermo Scientific™ Supplier No. 78442

Description

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

The all-in-one Thermo Scientific™ Halt Protease and Phosphatase Inhibitor Cocktail provides the convenience of a single solution with full sample protection.

Additional Information and Online Ordering

This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale.

Highlights:

  • Complete protection all-in-one cocktail contains both protease and phosphatase inhibitors

  • Multiple package sizes choose from four convenient packages sizes to match nearly any scale

  • Convenient the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats

  • Easy storage aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails

  • Compatible can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift

  • Validated fully compatible with Thermo Scientific Pierce™ Cell Lysis Buffers

Includes:

Formulations with and without supplementary vial of EDTA for metalloprotease inhibition, both of which are offered in four package sizes.

Specifications

Product Type Protease and Phosphatase Inhibitor Cocktail
Content And Storage Upon receipt store at 4°C
Form Liquid
Product Line Halt
Buffer Lysis Buffer
Reagent Type Protease Inhibitor, Phosphatase Inhibitor
Inhibitors Protease Inhibitor, Phosphatase Inhibitor
Quantity 24 x 100 μL

Frequently Asked Questions (FAQs)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.
What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.
Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.
How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

What formats are available for the combined Halt Protease and Phosphatase Inhibitor Cocktails?

The Halt Protease and Phosphatase Inhibitor Cocktails are offered as 100X concentrates in single-use or multiple-use formats. The metalloprotease inhibitor, EDTA, is provided in separate vials.

For Research Use Only. Not for use in diagnostic procedures.