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Cytiva GST Gene Fusion System pGEX Vectors

Catalog No. 45002271
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Kit Contents:
4T-1
4T-3
6P-1
6P-3
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Catalog No. Kit Contents
45002271 4T-1
45002277 6P-3
45002275 6P-1
45002282 4T-3
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Catalog No. 45002271 Supplier Cytiva Supplier No. 28954549
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Description

Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST

  • Offers a tac promoter for chemically inducible, high-level expression of GST-tagged recombinant proteins
  • Offers an internal laq1q gene for use in any E. coli host
  • With PreScission™ Protease, Thrombin, or Factor Xa recognition sites for cleaving the target protein from the fusion product
  • The vectors provide all three translational reading frames beginning with the EcoRI restriction site
  • Can use a wide variety of E. coli host strains cloning and expression

  • pGEX-1lT EcoRI/BAP is an EcoR1 pre cut vector, phosphatase treated to prevent self ligation, ready for gene insertion
  • pGEX-2TK will introduce a specific kinase site allowing the fusion protein to be labelled with [γ-32P]ATP using protein kinase

Specifications

Kit Contents 4T-1
Quantity 25 μg
For Use With (Application) Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schisto soma japonicum GST
Type pGEX Vector