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Gibco™ RPMI 1640 Medium (ATCC modification) DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer:  Gibco™ LSA1049101

Catalog No. A1049101



Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Modified by ATCC to contain high glucose, low sodium bicarbonate, sodium pyruvate, HEPES, low L-glutamine and phenol red
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses a sodium bicarbonate buffer system (1.5g/L) and therefore requires a 5-7% CO2 environment to maintain physiological pH

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature


RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.



Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Standard Serum Supplementation
Sodium Pyruvate
High Glucose
Room Temperature
Phenol Red

For Research Use Only. Not for use in diagnostic procedures.