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Thermo Scientific™ GeneJET RNA Purification Kit
Description
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
- Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
- Efficient—high yields of total RNA
- Fast—15 minutes after lysis step
- Pure—an A260/280 ratio > 1.9
- Convenient—spin columns are capped and assembled with collection tubes
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- RT-PCR
- RT-qPCR
- Northern blotting
- Nuclease protection assay
Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol
Specifications
Specifications
| Content And Storage | • 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening • 200 mL Lysis Buffer; room temperature • 200 mL Wash Buffer 1 (concentrated); room temperature • 100 mL Wash Buffer 2 (concentrated); room temperature • 125 mL Nuclease-free Water; room temperature • 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C. • 250 Collection Tubes (2 mL); room temperature • 250 Collection Tubes (1.5 mL); room temperature |
| Isolation Technology | Silica Spin Column |
| Purification Time | 15 min. (After sample homogenization) |
| Elution Volume | 50–100 μL |
| Sample Type | Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 250 Preps |
| Scale | Mini |
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Frequently Asked Questions (FAQs)
We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
We cannot recommend GeneJET kits for RNA purification from FFPE tissues. In case of genomic DNA purififcation from formalin-fixed paraffin-embedded samples, we recommend GeneJET FFPE DNA Purification Kit.
The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.
We recommend performing centrifugation steps at room temperature when the temperature is not specified.
No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.
For Research Use Only. Not for use in diagnostic procedures.