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Applied Biosystems™ GeneAmp™ 10X PCR Buffer

Catalog No. N8080006
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1.5 mL
6 x 1.5 mL
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N8080129 6 x 1.5 mL
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Description

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Applied Biosystems™ GeneAmp™ 10X PCR Buffer I is optimized for use with AmpliTaq™ DNA Polymerase, resulting in robust PCR amplification.

  • Optimized for use with AmpliTaq™ DNA Polymerase, offering maximum PCR performance
  • Available in several convenient sizes

GeneAmp™ 10X PCR Buffer I contains 15 mM MgCl2. When diluted 1:10, it provides the optimal pH and ionic strength for PCR amplification with AmpliTaq™ DNA Polymerase.

Note: See user's manual or package insert for limited label license and trademark information.

Order Info

Shipping Condition: Dry ice

Specifications

Chemical Name or Material PCR Buffer
Concentration 10X
For Use With (Application) Standard PCR
Quantity 1.5 mL
Includes • GeneAmp 10X PCR Buffer I (with 15 mM MgCl2), 1.5 mL

Store at -15°C to -25°C.

Frequently Asked Questions (FAQs)

What is BSA and its function in PCR? What concentration of BSA do you recommend?

BSA, bovine serum albumin, is usually used in PCR as a stabilizer. A concentration of 160 µg/mL is typical. Gelatin may also be used as a stabilizer at 0.01% (w/v).
What are the differences between GeneAmp 10X PCR Buffer I, PCR Buffer II and PCR Gold Buffer, and what are the formulations of each buffer?

The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been carefully optimized for use with the AmpliTaq Gold enzyme. GeneAmp 10X PCR Buffer I contains Mg; Geneamp 10X PCR Buffer II and Geneamp 10X PCR Gold Buffer do not have Mg in them, but come with a separate vial of 25 mM MgCl2 so that you can titrate the Mg in your PCR reaction.

Buffer formulations:
Buffer I (500 mM KCl, 100 mM Tris-HCl, pH 8.3, 15 mM MgCl2, 0.01% (w/v) gelatin)
Buffer II (500 mM KCl, 100 mM Tris-HCl, pH 8.3), with separate tube of 25 mM MgCl2
Gold Buffer (500 mM KCl, 150 mM Tris-HCl pH 8.0), with separate tube of 25 mM MgCl2

For Research Use Only. Not for use in diagnostic procedures.