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  6. Invitrogen™ FIX & PERM™ Cell Permeabilization Kit

Invitrogen™ FIX & PERM™ Cell Permeabilization Kit

Catalog No. GAS004
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GAS004 200 Tests
GAS003 50 Tests
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Catalog No. GAS004 Supplier Invitrogen™ Supplier No. GAS004
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Description

FIX & PERM™ Cell Permeabilization Kit

The FIX & PERM™ Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.

The FIX & PERM™ Cell Permeabilization Kit is:

  • Flexible—compatible with multiple sample types and cellular antigens
  • Robust—scatter pattern of cells unaffected
  • Efficient—simultaneous detection of intracellular and surface markers

    View a selection guide for all available FIX & PERM™ products and products related to sample preparation (including cell lysis and preservation solutions, blocking reagents, and information about the use of Dynabeads™ cell separation products for cell isolation).

    The FIX & PERM™ formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.The FIX & PERM™ Cell Permeabilization Kit is suitable for various biological samples and has been validated for use with cell lines, whole blood, and bone marrow. The FIX & PERM™ kit has been shown to accurately identify previously undetectable intracellular markers. Unlike other fixation and permeabilization reagents available on the market, the FIX & PERM™ staining protocol leaves the morphological scatter characteristics of the cells intact.

    How It Works
    The FIX & PERM™ Cell Permeabilization Kit contains 2 reagents: Fixation Medium (Medium A) and Permeabilization Medium (Medium B). Cells are incubated for 15 minutes at room temperature with Fixation Medium, washed, and then incubated with the Permeabilization Medium and fluorochrome-labeled antibody. Fixation Medium and Permeabilization Medium are also available separately (Cat. Nos. GAS001S100 & GAS002S100).

    • Specifications

    Product Line FIX & PERM
    Quantity 200 tests
    Product Type Fixation/Permeabilization Reagent
    No. of Tests 200 Tests
    Shipping Condition Room Temperature
    Content And Storage Store at room temperature.

    Frequently Asked Questions (FAQs)

    Is the FIX & PERM Cell Permeabilization Kit (Cat. No. GAS003, GAS004) compatible with conjugates (dyes) besides FITC and PE?

    Yes, this product is compatible with other conjugated antibodies, outside of FITC and PE.
    The FIX & PERM Cell Permeabilization Kit protocol does not say what volume my cells should be in when they are added to the tube. What should I do?

    Your cells should be in approximately 100 µL volume. One way to do this is to aliquot the correct number of cells (10E6) and then spin them down and decant the supernatant. The residual volume should be approximately 100 µL.

    Can I use the FIX & PERM Cell Permeabilization Kit to access nuclear epitopes?

    Yes. Generally, you need to increase the incubation time in the permeabilization buffer (Reagent B). Here are a couple of references:
    -Millard I, Degrave E, Philippe M et al. (1998) Detection of intracellular antigens by flow cytometry: comparison of two chemical methods and microwave heating. Clin Chem 44:2320-2330(http://www.ncbi.nlm.nih.gov/pubmed/9799760).
    -Parker JE, Mufti GJ, Rasool F et al. (2000) The role of apoptosis, proliferation, and the Bcl-2-related proteins in the myelodysplastic syndromes and acute myeloid leukemia secondary to MDS. Blood 96:3932-3938(http://www.ncbi.nlm.nih.gov/pubmed/11090080).
    The FIX & PERM Cell Permeabilization Kit is used to prepare cells for flow cytometry; can I use it for immunohistochemistry?

    Yes. Here are a couple of references:
    Haranaga S, Yamaguchi H, Friedman H et al.(2001) Chlamydia pneumoniae infects and multiplies in lymphocytes In vitro. Infect Immun 69:7753-7759 (http://www.ncbi.nlm.nih.gov/pubmed/11705957).

    Al-Mufti R, Hambley H, Albaiges G et al. (2000) Increased fetal erythroblasts in women who subsequently develop pre-eclampsia. Hum Reprod 15:1624-1628 (http://www.ncbi.nlm.nih.gov/pubmed/10875878).
    Does the FIX & PERM Cell Permeabilization Kit (Cat. Nos. GAS003 and GAS004) lyse red blood cells?

    Yes. The FIX & PERM Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics. It has been validated for use in cell lines, whole blood, and bone marrow. In addition fixing and permeabilizing cells, it lyses red blood cells. It is labeled as a general purpose reagent (GPR) and can be used in clinical settings, but it is also used widely in research labs.

    For Research Use Only. Not for use in diagnostic procedures.