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Thermo Scientific™ ZipRuler Express DNA Ladder Set, ready-to-use
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Catalog No. FERSM1373
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2 x 50 μg
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FERSM1373 2 x 50 μg
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Catalog No. FERSM1373 Supplier Thermo Scientific™ Supplier No. SM1373

Description

Perform sizing and approximate quantification of wide range of double-stranded DNA for fast and long electrophoresis runs.

ZipRuler Express DNA Ladder Set contains two ladders: ZipRuler Express DNA Ladder 1 and ZipRuler Express DNA Ladder 2. Both are mixtures of chromatography-purified individual DNA fragments.The set is designed for fast and highly accurate sizing of a broad range (100-20,000 bp) of DNA fragments and their approximate quantification under a variety of electrophoresis conditions. The band pattern is not affected by the composition of electrophoresis buffer, voltage or gel percentage (see Table 1).

Highlights

  • Flexible -can be run in two lanes or combined in one lane
  • Composed of chromatography purified individual DNA fragments
  • Ready-to-use -premixed with a loading dye for direct loading and room temperature storage
  • Supplied with loading dye for sample DNA

Specifications

Concentration 6X
Gel Type Agarose
Label or Dye Xylene Cyanol FF, Orange G, Bromophenol Blue
Ready to Load Yes
Size Range 100 to 20000 bp
Gel Compatibility Agarose Gels
Quantity 2 x 50 μg
Product Line ZipRuler
Product Type DNA Ladder Set

Frequently Asked Questions (FAQs)

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.
Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.
What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.

For Research Use Only. Not for use in diagnostic procedures.