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Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.

$59.06 - $235.20

Specifications

Concentration 10U/µL
Inhibitors metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50mM
Molecular Weight The enzyme is a homotetramer. It consists of four identical subunits of 28.9 kDa
Source E. coli cells with a cloned pseT gene of bacteriophage T4
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Products
Catalog Number Mfr. No. Units Price Quantity    

FEREK0031

 
thermo scientific™
EK0031
500U Each for $59.06

FEREK0032

 
thermo scientific™
EK0032
2,500U Each for $235.20
Description & Specifications

Specifications

Concentration 10U/µL
Inhibitors metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50mM
Molecular Weight The enzyme is a homotetramer. It consists of four identical subunits of 28.9 kDa
Source E. coli cells with a cloned pseT gene of bacteriophage T4

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction).

The enzyme is also a 3'-phosphatase.

Highlights

  • Active in Thermo Scientific restriction enzyme, RT, and T4 DNA Ligase buffers