T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction).
The enzyme is also a 3'-phosphatase.
- Active in Thermo Scientific restriction enzyme, RT, and T4 DNA Ligase buffers
|metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50mM|
|The enzyme is a homotetramer. It consists of four identical subunits of 28.9 kDa|
|E. coli cells with a cloned pseT gene of bacteriophage T4|