missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. Thermo Scientific™ SspI (10 U/μL)

Thermo Scientific™ SspI (10 U/μL)

Numéro de catalogue. FERER0771
Change view
Click to view available options
Quantity:
2,500 units
500 units
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantity
FERER0771 500 units
FERER0772 2,500 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Numéro de catalogue. FERER0771 Fournisseur Thermo Scientific™ Code fournisseur ER0771
Il en reste null

Description

The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer.

Lambda DNA digested with SspI, 1% agarose, 20 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...A A T▵A T T...3'

3'...T T A▵T A A...5'

Conditions for 100% Activity

  • 1X Buffer G:10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA.Incubate at 37°C

Storage Buffer

  • SspI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 10-fold overdigestion with SspI, more than 90% of the DNA fragments can be ligated and recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — no effect

Digestion of Agarose-embedded DNA

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Greater than 10-fold overdigestion with SspI may result in star activity.

Spécifications

Concentration 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme Ssp I
Compatible Buffer 10X Buffer G
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 500 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.