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Thermo Scientific™ RiboRuler High Range RNA Ladder, ready-to-use

Catalog No. FERSM1823
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FERSM1823 5 x 40 μL
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Catalog No. FERSM1823 Supplier Thermo Scientific™ Supplier No. SM1823
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Description

Perform RNA sizing and quantification on native or denaturing gels with this ready-to-use RNA Ladder composed of 8 chromatography purified single-stranded RNA transcripts.

RiboRuler High Range RNA Ladder 200 to 6000 bases, ready-to-use is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments. Ladder is premixed with loading dye.

RiboRuler High Range RNA Ladder, ready-to-use, is composed of chromatography-purified transcripts that makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use, the ladder is aliquotted in smaller volumes.

Highlights

  • Sharp bands of uniform intensity
  • Easy-to-remember band sizes and quantities
  • Predetermined concentration of ladder bands allows for approximate RNA quantification
  • Supplied with 2X RNA Loading Dye
  • Stable for 6 months at -20°C

Specifications

Concentration 2X
Gel Type Agarose
Label or Dye Xylene Cyanol FF, Bromophenol Blue, Ethidium Bromide
Size Range 200 to 6000 bp
Quantity 5 x 40 μL
Product Line RiboRuler
Product Type High Range DNA Ladder

Frequently Asked Questions (FAQs)

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.
Can I use ready-to-use RiboRuler RNA Ladders in native gel electrophoresis?

Ready-to-use RiboRuler RNA Ladders are not suitable for native gel electrophoresis since they are in a loading buffer that contains 47.5% formamide and 0.0125% SDS.
What ladder do you recommend for running RNA on Invitrogen E-Gel EX Agarose Gels?

Depending on the RNA fragment size, we recommend the following ladders:

  • RiboRuler Low Range RNA ladder, (100 bp-1,000 bp) (Cat. No. SM1833)

  • RiboRuler High Range RNA ladder, (200 bp-6,000 bp) (Cat. No. SM1823)

For Research Use Only. Not for use in diagnostic procedures.