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Thermo Scientific™ RevertAid™ Premium First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

$100.01 - $396.90

Specifications

For Use With (Application) First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.
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Products
Catalog Number Mfr. No. Sufficient For Price Quantity    

FERK1621

 
thermo scientific™
K1621
20 reactions Each for $100.01

FERK1622

 
thermo scientific™
K1622
100 reactions Each for $396.90
Description & Specifications

Specifications

For Use With (Application) First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.

RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Highlights

  • Full-length first strand cDNA up to 13 kb
  • Optimum reaction temperature 42°C
  • Complete kit -all the components for the RT reaction are included