missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Please login to your online account to display your discounted pricing
Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Supplier: Thermo Scientific™ K0253
Description
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.
- Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity - detects low copy number targets
- Wide linear range - accurate quantification across 9 orders of magnitude
- Reproducibility and convenience - ready-to-use 2X master mix
Specifications
| Contains: • 4 x 12.5 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 4000 x 25 μL reactions. • 1 mL ROX solution, 50 μM • 2 x 30 mL Nuclease-Free Water Store at -20°C. |
|
| Tube | |
| qPCR | |
| Standard | |
| Maxima | |
| 4000 Reactions of 25 μL | |
| 2X | |
| DNA, dsDNA |
| SYBR | |
| High | |
| Taq DNA Polymerase | |
| BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene | |
| Real Time PCR SYBR Master Mix | |
| Gene Expression | |
| 4,000 Reactions | |
| 4 x 12.5 mL |
For Research Use Only. Not for use in diagnostic procedures.