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Thermo Scientific™ GeneRuler Express DNA Ladder
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Catalog No. FERSM1551
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50 μg
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FERSM1551 50 μg
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Catalog No. FERSM1551 Supplier Thermo Scientific™ Supplier No. SM1551
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Description

Thermo Scientific™ Fermentas GeneRuler Express DNA Ladder is designed for fast separation (in 5 to 15 min. at 23V/cm) of double-stranded DNA fragments in agarose gels.

Perform fast separation (in 5 to 15 min. at 23V cm) of double-stranded DNA fragments in agarose gels with Thermo Scientific™ Fermentas GeneRuler™ Express DNA Ladder.

The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments. GeneRuler Express DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase, protocol. The ladder is supplied with 6X DNA Loading Dye for sample DNA.
  • Ideal for both DNA sizing and approximate quantification
  • Ideal for fast separation under a wide range of electrophoresis conditions
  • Sharp bands
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA

Specifications

Concentration 6X
Gel Type Agarose
Label or Dye Xylene Cyanol FF, Bromophenol Blue, Orange G
Ready to Load No
Size Range 100 to 5000 bp
Gel Compatibility Agarose Gels
Quantity 50 μg
Product Line GeneRuler
Product Type DNA Ladder

Frequently Asked Questions (FAQs)

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.
Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.
What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.
When using a NanoDrop spectrophotometer, what is the difference between controls versus standards?

NanoDrop CF-1 Calibration Fluid is the only photometric standard available to assess the accuracy of the NanoDrop spectrophotometer pedestal. If the control sample shows unexpected results the user should confirm the calibration of the instrument with the CF-1 fluid.
Some control examples include:
- Nucleic Acids: Fermentas GeneRuler Express DNA Ladder 100-5000 bp (Cat. No. SM1551)
- Protein: Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209)

For Research Use Only. Not for use in diagnostic procedures.