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Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.
Supplier: Thermo Scientific™ K0491
Description
Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers greater than 400 ng/μL of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights
- Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
- High concentration of DNA purified— >400 ng/μL
- Convenient—spin column format, centrifuge or vacuum protocols
- Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- Transfection of robust cell lines
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription
Specifications
| 400 ng/μL | |
| Spin Column | |
| Maxi | |
| Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR | |
| 10 Preps | |
| GeneJET | |
| 10 preps | |
| 60 min. |
| Spin Column | |
| Silica Column | |
| Bacteria | |
| Not High-throughput Compatible (Manual) | |
| > 200 μg (Large-Scale) Plasmid DNA | |
| Plasmid MaxiPrep Kit | |
| Plasmid DNA |
For Research Use Only. Not for use in diagnostic procedures.