Recombinant Protein;50UG;Human CD32b, His Tag (SPR& BLI verified) (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.;Immobilized Human CD32b, His Tag (SPR& BLI verified) (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 10.1 M as determined in a SPR assay (Biacore T200) (QC tested). ;Immobilized Rituximab on CM5 Chip, can bind Human CD32b, His Tag (SPR& BLI verified) (Cat. No. CDB-H5228) with an affinity constant of 5.5 M as determined in a SPR assay (Biacore T200) (Routinely tested).
