missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Secondary Antibodies
  5. Rabbit Secondary Antibodies
  6. SA510394

Invitrogen™ F(ab)-Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody

Catalog No. SA510394
Change view
Click to view available options
Quantity:
250 μL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
SA510394 250 μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. SA510394 Supplier Invitrogen™ Supplier No. SA510394

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

F(ab)-Rabbit Recombinant Superclonal Secondary Antibody

F(ab)-Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody detects both the Fab2 and Fc fragments of Goat IgG. It showed no cross-reactivity to various species immunoglobulins tested. This Fab fragment antibody contains a hexa-histidine tag, is expressed in vitro and purified as a recombinant construct. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Goat IgG (H+L)
Applications ELISA, Western Blot, Immunocytochemistry
Classification Recombinant Superclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.4
Host Species Rabbit
Immunogen Purified Goat IgG.
Purification Method Affinity chromatography
Quantity 250 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Goat
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Secondary Antibody
Form Liquid
Isotype IgG F(ab)
Show More Show Less