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Invitrogen™ eBioscience™ eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit

Catalog No. 501125101
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501125101 1 Kit
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Contains Sodium azide and formaldehyde
Catalog No. 501125101 Supplier Invitrogen™ Supplier No. 8822685441
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Contains Sodium azide and formaldehyde

Description

Includes

  • eZKine CD8 Activation 1 Cocktail : 25 tests. This cocktail contains the following antibodies:
  • Anti-Human CD3 (SK7) APC
  • Anti-Human CD8 (SK1) PerCP-eFluor 710
  • Anti-Human CD69 (FN50) PE
  • Anti-Human IFN gamma (4S.B3) FITC
  • eZKine Isotype Control Cocktail D : 25 tests. This cocktail contains the following antibodies:
  • Anti-Human CD3 (SK7) APC
  • Anti-Human CD8 (SK1) PerCP-eFluor 710
  • Mouse IgG1 K Isotype Control PE
  • Mouse IgG1 K Isotype Control FITC
  • eZKine Fix/Lyse Concentrate (4X)
  • eZKine Fix/Lyse Diluent
  • Permeabilization Buffer (10X)

Designed to rapidly identify cytokine producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples.

  • Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization.
  • Samples are then ready to stain with the CD8 Activation 1 cocktail and concentration-matched Isotype Control cocktail.

Specifications

Reactivity Human
Detects CD3, CD69, CD8, IFN gamma
For Use With (Application) Intracellular Staining Followed by Flow Cytometric Analysis
Quantity 1 Kit
Content And Storage 2°C to 8°C. Do not freeze. Light sensitive material.