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MilliporeSigma™ EZ-Zyme™ Chromatin Prep Kit
ChIP analysis at nucleosome resolution by performing complete or partial digestions with a proprietary enzymatic cocktail to obtain chromatin fragments of on average one to a few nucleosomes in length
Supplier: MilliporeSigma™ 17375
Includes:
Store at 4°C:
- 0.5M EDTA
- 5M NaCl
- 1M Tris-HCl, pH 6.5
- 10X Glycine
- 10X PBS
- EZ-Zyme™ Lysis Buffer
- EZ-Zyme™ Digestion Buffer
- EZ-Zyme™ Stop Buffer
Store at -20°C:
- Protease Inhibitor Cocktail II.
- RNase A
- Proteinase K
- EZ-Zyme™ Enzymatic Cocktail
Requires :
- Cells, stimulated or treated as needed for the experimental system
- 37% Formaldehyde
- Liquid nitrogen (N2 ) or dry ice/alcohol bath
- Note: care should be exercised in handling liquid nitrogen as liquid can damage skin or tissue on contact. Use appropriate personal protective equipment when working with liquid nitrogen or the dry ice/alcohol bath
Description
- ChIP analysis at nucleosome resolution by performing complete or partial digestions with a proprietary enzymatic cocktail to obtain chromatin fragments of on average one to a few nucleosomes in length.
- Compared to sonication, this procedure not only allows mapping the protein-DNA association at a higher resolution, but also renders the subsequent immunoprecipitation more efficient due to its mild digestion condition.
- In addition, enzymatic shearing is compatible with non-crosslinked ChIP (native ChIP), which allows analysis of histone modifications not only on cultured cells, but also on freshly dissected and frozen tissues
Specifications
| Store components at the temperatures indicated on the labels | |
| Enzymatic shearing of chromatin from mammalian cells at higher resolution than sonication for use in chromatin immunoprecipitation (ChIP) assays | |
| 1 Kit | |
| Stable for 6 months from date of shipment when stored as directed |