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MilliporeSigma™ EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit

Catalog No. 1710461
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24 Assays
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Catalog No. Quantity
1710461 24 Assays
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Catalog No. 1710461 Supplier MilliporeSigma™ Supplier No. 1710461
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Description

Includes

  • 10X Glycine
  • Nuclei Isolation Buffer
  • 10X PBS
  • SCW Buffer (Sonication/ChIP/Wash)
  • Magna ChIP Protein A/G Magnetic Beads
  • Low Stringency IP Wash Buffer
  • ChIP Elution Buffer
  • Protease Inhibitor Cocktail
  • Proteinase K Solution
  • Anti-Trimethyl-Histone H3 (Lys4)
  • Normal Rabbit IgG
  • Control PCR primers

Requires

Reagents

  • Cells, stimulated or treated as desired
  • Antibody of interest for chromatin immunoprecipitation
  • 37% Formaldehyde
  • qPCR reagents, such as SYBR™ Green Master Mix
  • 2% Agarose gel
  • 100 bp DNA Ladder
  • 6X gel Loading buffer
  • DNase- and RNase-free sterile H2O

Specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipitation and enables the use of a broader range of antibodies, eliminating the need to purchase different kits for different antibody isotypes.

  • Robust ChIP from as few as 10,000 to as many as 1,000,000 cells
  • Protocols and reagents for generation of chromatin from range of input sample type
  • Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies
  • Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment
  • Single buffer system for sonication, chromatin IP, and wash
  • Perform analysis of enrichment without additional purification after cross link reversal
  • Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray
  • Includes positive and negative control antibodies and assay against known positive locus

Specifications

Content And Storage Store components at the temperatures indicated on the labels
For Use With (Application) To enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads
Quantity 24 Assays
Stability Kit components are stable for 1 year from date of shipment when stored as directed