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Thermo Scientific™ EZ-Link™ HPDP-Biotin, No-Weigh™ Format
Supplier: Thermo Scientific™ 21341
Description
Thermo Scientific™ EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods.
Highlights:
- Pyridyldithiol reacts with -SH groups to form a stable disulfide bond
- Absorbance of pyridine-2-thione leaving group at 343nm can be used to determine reaction progress
- Disulfide bond is cleavable using 50mM DTT (Part No. 20291) or 100mM β-Mercaptoethanol (Part No. 35600)
- Extended spacer arm prevents steric hindrance
- Must be dissolved in DMSO or DMF before use
- Biotinylation occurs between pH 6-9
Specifications
| HPDP-Biotin | |
| Store at 4°C. | |
| Cell-Permeant | |
| Pyridyldisulfide | |
| Biotin | |
| Biotin & Analogs |
| 50 mg | |
| EZ-Link | |
| Thiol | |
| DMF (Dimethylformamide), DMSO (Dimethylsulfoxide) | |
| Long, Cleavable |
For Research Use Only. Not for use in diagnostic procedures.