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Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.

Supplier: Invitrogen™ 1178101K

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Catalog No. 1178101K

$8,360.00 / Each

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Description

Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments
This fast, one-step mix is designed to work with a variety of instruments’ default protocols, including Applied Biosystems and non-Applied Biosystem (see universal version) qPCR platforms
Fast-activating, antibody-mediated Platinum Taq DNA Polymerase for complete, rapid activation; provides high sensitivity and reproducibility
The One-Step SuperScript III Reverse Transcriptase module is a unique formulation to ensure high yield and less PCR inhibition
Flexible formats with separate or pre-mixed ROX are designed to work with default instrument protocols on a wide range of high-throughput fast instruments
Contains heat-labile UDG which significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C
Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4°C to 8°C
Store at -20°C for long-term storage

Specifications

Content And Storage	The EXPRESS One-Step SuperScript™ qRT-PCR Universal kit contains 3 modules: 2X EXPRESS SuperScript™ Mix (5 × 1 mL), Express qPCR SuperMix Universal (5 × 5 mL), and ROX Reference Dye (5 × 500 μL). Store at -20°C for long-term storage. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4–8°C.
Format	Tube
Polymerase	Taq DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Product Type	qRT-PCR Kit
Sufficient For	2500 Reactions
Sample Type	RNA

Detection Method	Primer-probe
PCR Method	1-step RT-qPCR
Reaction Speed	Fast or Standard
Product Line	SuperScript™
Quantity	2,500 reactions
No. of Reactions	2500 Reactions

Product Suggestions Documents

Documents

Product Information

Manuals

EXPRESS One-Step SuperScript qRT-PCR Kits Version: 28 June 2010 File size: 342.3 kB

For Research Use Only. Not for use in diagnostic procedures.