Thermo Scientific™ Exonuclease I (20 U/μL)

Name: Thermo Scientific&trade; Exonuclease I (20 U/&mu;L)
Brand: Thermo Scientific&trade;
SKU: FEREN0581
Price: 143.00 CAD

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Supplier: Thermo Scientific™ EN0581

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Catalog No. FEREN0581

$143.00 / Each

Quantity

Description

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

Active in PCR buffers

Applications

Primer removal from PCR mixtures:
- prior to PCR product sequencing
Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

Specifications

Concentration	20 U/μL
Enzyme	Exonuclease
Quantity	4000 U
Product Type	Exonuclease I

For Research Use Only. Not for use in diagnostic procedures.