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MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys27), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Numéro de catalogue. 17622MI
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Quantité:
25 Assays
Conjugué:
Unconjugated
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Numéro de catalogue. Quantité Conjugué
17622MI 25 Assays Unconjugated
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Numéro de catalogue. 17622MI Fournisseur MilliporeSigma™ Code fournisseur 17622

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Description

Rabbit Polyclonal Antibody

Specifically detects Trimethyl-Histone H3 (Lys27) Clone: in Human, Mouse samples, and it is validated for ChIP, Western Blotting

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Spécifications

Antigène Trimethyl-Histone H3 (Lys27)
Applications ChIP Assay, Western Blot
Classification Polyclonal
Conjugué Unconjugated
Formule Anti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100μg protein A purified IgG in 100μL buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg purified Rabbit IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers human alpha-Satellite. One vial containing 75μL of 5μM of each control primer specific for human alpha-Satellite. FOR: CTG CAC TAC CTG AAG AGG AC; REV: GAT GGT TCA ACA CTC TTA CA
Numéro d’acquisition du gène Q16695
Symboles de gène H3F3A, MGC87783, H3.3A, MGC87782, H3F3, H3.3B, H3F3B
Espèces hôtes Rabbit
Immunogène KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.
Quantité 25 Assays
Statut réglementaire RUO
Disciplines de recherche Epigenetics and Nuclear Function
Primaire ou secondaire Primary
ID du gène (Entrez) NP_003484
Spécifications de test trimethyl-Histone H3 (Lys27)
Comprend Trimethyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Me3.
Espèces cibles Human, Mouse
Contenu et stockage −20°C for one year
Forme Purified
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For Research Use Only