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MilliporeSigma™ PvuRts1I (5-hmC Restriction Endonuclease)
PvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosylated and non-glucosylated 5-hmC DNA sequences.
Supplier: MilliporeSigma™ 141058
Specifications
| PvuRts1I 5-hmC Endonuclease: 100U (1U/μL) in a buffer containing 20mM Tris pH 8.0, 150mM NaCl, 1mM DTT and 50% Glycerol. PvuRts1I 5-hmC 10X Reaction Buffer: 500μL of buffer containing 200mM Tris pH 8.0, 1.5M NaCl, 50mM MgCl2 and 10mM DTT. | |
| -20°C for 12 months. | |
| PvuRts1I (5-hmC Restriction Endonuclease) |
| Analyzing 5-hmC patterns genomic DNA by cleaving both glucosylated and non-glucosylated 5-hmC DNA sequences | |
| 200 U |