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MilliporeSigmaâ„¢ Immobilon Western HRP Chemiluminescence Substrates
Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns
$205.20 - $621.12
| Catalog Number | Mfr. No. | Product Type | Quantity | Sensitivity | Price | Quantity | |||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Product Type | Quantity | Sensitivity | Price | Quantity | |||
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WBLUF0500
|
MilliporeSigmaâ„¢
WBLUF0500 |
HRP Substrate | 500 mL | ∼400 fg |
N/A
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WBLUR0500
|
MilliporeSigmaâ„¢
WBLUR0500 |
HRP Substrate | 500 mL | ∼1 to 3 pg |
N/A
|
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WBLUF0100A
|
MilliporeSigmaâ„¢
WBLUF0100 |
HRP Substrate | 100 mL | ∼400 fg |
N/A
|
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WBLUC0500
|
MilliporeSigmaâ„¢
WBLUC0500 |
HRP Substrate | 500 mL | ∼6 pg |
N/A
|
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Description
Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.
- Consistent results with less pipetting error
The stabilizers added to Luminata substrates:
- Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
- Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
- Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation
Three different sensitivities for a range of detection needs:
- Immobilon Classico premixed reagent for everyday detection needs
- Immobilon Crescendo premixed reagent for detection in the picogram range
- Immobilon Forte premixed reagent for detection in the femtogram range
Specifications
| HRP Substrate | |
| Dot/Slot Blot |