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MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates
Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns
$148.00 - $442.00
| Catalog Number | Mfr. No. | Product Type | Quantity | Sensitivity | Price | Quantity | |||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Product Type | Quantity | Sensitivity | Price | Quantity | |||
| WBLUF0100A |
MilliporeSigma™
WBLUF0100 |
Luminata Forte Western HRP Substrate | 100mL | ∽400fg |
Each for $148.00
|
|
WBLUC0500 |
MilliporeSigma™
WBLUC0500 |
Luminata Classico Western HRP Substrate | 500mL | ∽6pg |
Each for $223.00
|
|
WBLUF0500 |
MilliporeSigma™
WBLUF0500 |
Luminata Forte Western HRP Substrate | 500mL | ∽400fg |
Each for $442.00
|
|
WBLUR0500 |
MilliporeSigma™
WBLUR0500 |
Luminata Crescendo Western HRP Substrate | 500mL | ∽1 to 3pg |
Each for $318.00
|
|
Description
Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.
- Consistent results with less pipetting error
The stabilizers added to Luminata substrates:
- Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
- Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
- Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation
Three different sensitivities for a range of detection needs:
- Luminata Classico premixed reagent for everyday detection needs
- Luminata Crescendo premixed reagent for detection in the picogram range
- Luminata Forte premixed reagent for detection in the femtogram range
Specifications
| Western blotting, dot/slot blotting | |
| HRP |