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Edvotek™ Sickle Cell Gene Detection 
Gain an understanding of the effect of mutations on health and disease.
Supplier: Edvotek™ 116
Includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets
Requires : Horizontal gel electrophoresis apparatus; D.C. power supply; Automatic micropipets with tips; Balance; Microwave, hotplate or burner; Pipet pump; 250mL flasks or beakers; Hot gloves; Safety goggles and disposable laboratory gloves; Small plastic trays or large weigh boats (for gel destaining); DNA visualization system (white light); Distilled or deionized water
Description
- Genetically inherited diseases in humans can be caused by a single nucleotide mutation resulting in a change. Such a substitution can affect the structure and function of a critical protein, rendering it partially or completely nonfunctional. A classic example is hemoglobin in sickle cell anemia
- Agarose gel-based experiment designed for DNA staining and visualization with InstaStain™ Methylene Blue
- Demonstrates the use of a restriction enzyme that discriminates between the normal and sickle cell hemoglobin genes
- This experiment does not contain human DNA
Specifications
| 9 to Undergraduate | |
| Instructions; ready-to-load™ quickstrip™ dna samples; ultraspec agarose™ powder; practice gel loading solution; electrophoresis buffer; instastain™ blue and flashblue™ | |
| Electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system | |
| Refrigerated | |
| Sickle-cell Gene Detection (DNA-based) Classroom Kit |
| To investigate the restriction enzyme that discriminates between hba (normal) and hbs (disease) genes and perform a simulated test on a patient | |
| 8 Gels | |
| 45 min. | |
| 8 Lab Groups |