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Edvotek™ Mapping of Restriction Sites on Plasmid DNA 
Perform electrophoresis of plasmid DNA in parallel with standard DNA markers.
Supplier: Edvotek™ EDV105
Includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets.
Requires :
- Horizontal electrophoresis apparatus
- DC power supply
- Staining tray and net
- Visualization system (white light)
- Automatic micropipets with tips to obtain sample for five gels
- Pipet pump
- 250mL Flasks
- Distilled or deionized water
- Water bath (65°C)
- Hotplate, Bunsen burner or microwave
- Balance
Description
- In this experiment, plasmid DNA is mapped after the electrophoresis of plasmid DNA fragments in parallel with standard DNA markers
- Teach students that DNA mapping is a common procedure used to determine the location of genes
- Also show that genes can be excised if students know the location of specific restriction sites within DNA
Specifications
| 9 to Undergraduate | |
| Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, and Disposable Pipets | |
| 8 Lab Groups |
| Electrophoresis of plasmid DNA in parallel with standard DNA markers | |
| 5 Gels (4 Lanes Each) | |
| Electrophoresis Kit |