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Edvotek™ Mapping of Restriction Sites on Plasmid DNA

Perform electrophoresis of plasmid DNA in parallel with standard DNA markers.

Supplier:  Edvotek™ EMD105

Catalog No. S10105



Includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets.

Requires :
  • Horizontal electrophoresis apparatus
  • DC power supply
  • Staining tray and net
  • Visualization system (white light)
  • Automatic micropipets with tips to obtain sample for five gels
  • Pipet pump
  • 250mL Flasks
  • Distilled or deionized water
  • Water bath (65°C)
  • Hotplate, Bunsen burner or microwave
  • Balance


Description

Description

  • In this experiment, plasmid DNA is mapped after the electrophoresis of plasmid DNA fragments in parallel with standard DNA markers
  • Teach students that DNA mapping is a common procedure used to determine the location of genes
  • Also show that genes can be excised if students know the location of specific restriction sites within DNA

Specifications

Specifications

Electrophoresis of plasmid DNA in parallel with standard DNA markers
Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, and Disposable Pipets
5 Gels (4 Lanes Each)
SDS
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