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Edvotek™ Mapping of Restriction Sites on Plasmid DNA

Catalog No. S10105
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8 Lab Groups
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Catalog No. Class Size
S10105 8 Lab Groups
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Catalog No. S10105 Supplier Edvotek™ Supplier No. EDV105
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Description

Includes

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets.

Requires

  • Horizontal electrophoresis apparatus
  • DC power supply
  • Staining tray and net
  • Visualization system (white light)
  • Automatic micropipets with tips to obtain sample for five gels
  • Pipet pump
  • 250mL Flasks
  • Distilled or deionized water
  • Water bath (65°C)
  • Hotplate, Bunsen burner or microwave
  • Balance

Perform electrophoresis of plasmid DNA in parallel with standard DNA markers.

  • In this experiment, plasmid DNA is mapped after the electrophoresis of plasmid DNA fragments in parallel with standard DNA markers
  • Teach students that DNA mapping is a common procedure used to determine the location of genes
  • Also show that genes can be excised if students know the location of specific restriction sites within DNA

Specifications

Grade 9 to Undergraduate
For Use With (Application) Electrophoresis of plasmid DNA in parallel with standard DNA markers
Includes Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, and Disposable Pipets
Quantity 5 Gels (4 Lanes Each)
Class Size 8 Lab Groups
Product Type Electrophoresis Kit