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Applied Biosystems™ DS-33 GeneScan™ Installation Standards with GeneScan™ 600 LIZ™ Size Standard v2.0

Catalog No. 4376911
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Catalog No. 4376911 Supplier Applied Biosystems™ Supplier No. 4376911
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Description

Includes

Each kit contains four tubes of GeneScan™ Installation Standard DS-33 and one tube of GeneScan™ 600 LIZ™ size standard v2.0.

The DS-33 GeneScan™ Installation standard consists of pooled PCR products labeled with 6-FAM™, VIC™, NED™ and PET™ dyes.

The DS-33 GeneScan™ Installation Standard consists of pooled PCR products labeled with 6-FAM™, VIC™, NED™ and PET™ dyes. To generate the pooled products, Human DNA has been amplified with 8 fluorescent PCR primer pairs which amplify selected microsatellite loci (as listed in the package product insert). The kit also contains a tube of GeneScan™ 600 LIZ™ Size Standard 2.0.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Order Info

Kit

Specifications

Content And Storage Each kit contains four tubes of GeneScan™ Installation Standard DS-33 and one tube of GeneScan™ 600 LIZ™ size standard v2.0.

Store the DS-33 tubes at -25° to -15°C

Store the GeneScan 600 LIZ Size Standard v2.0 opon thawing at 2° to 8°C, protect from light.
Format Tube
For Use With (Equipment) 3730xl DNA Analyzer, SeqStudio Genetic Analyzer, 3500 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer
Label or Dye FAM, LIZ, NED, PET, VIC
Product Line GeneScan, LIZ
Product Type Installation Standard
Quantity 1 Kit
Shipping Condition Dry Ice
Test Time Standard

Frequently Asked Questions (FAQs)

What does the SeqStudio Starter Kit (PN A35000) contain?

The SeqStudio Starter Kit (PN A35000) includes:
- SeqStudio Integrated Capillary Protector (Cat. No. A31923)
- SeqStudio Cathode Buffer Container (Cat. No. A33401)
- Sequencing Standard, BigDye Terminator v3.1, for 3500/SeqStudio Flex (Cat. No. 4404312)
- DS-33 GeneScan Installation Standards with GeneScan 600 LIZ Size Standard v2.0 (Cat. No. 4376911)
- Hi-Di Formamide (Cat. No. 4401457)
- MicroAmp Optical 96-Well Reaction Plate with Barcode (Cat. No. 4306737)
- MicroAmp 96-Well Tray/Retainer Set (Cat. No. 403081)
- MicroAmp Optical 8-Tube Strip, 0.2 mL (Cat. No. 4316567)
- Nuclease-Free Water (not DEPC-Treated) (Cat. No. AM9930)
- Cathode Buffer Container Reservoir Septa for SeqStudio Genetic Analyzer (Cat. No. A35640)
- 96-well Septa for SeqStudio Genetic Analyzer (Cat. No. A35641)
- 8-strip Septa for SeqStudio Genetic Analyzer (Cat. No. A35643)
- SeqStudio Cartridge v2 (PN A41328) (if this component is ordered online the catalog number is A41331)
When I started my run on the Applied Biosystems 3500/3500xL Genetic Analyzer, an error occurred: "Unstable Electrophoresis current detected, check for air bubbles" but I don't see any bubbles. What else can cause this?

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Running Buffer that was:
-On the instrument longer than 2 weeks
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.
Do I need to replace the buffer every 14 days if the Applied Biosystems 3500/3500xL Genetic Analyzer is not in use?

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer containers prior to starting a new run.

For Research Use Only. Not for use in diagnostic procedures.