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  6. Thermo Scientific™ DNA Polymerase I (10 U/μL)

Thermo Scientific™ DNA Polymerase I (10 U/μL)

Catalog No. FEREP0041
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500 units
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FEREP0041 500 units
FEREP0042 2,500 units
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Catalog No. FEREP0041 Supplier Thermo Scientific™ Supplier No. EP0041
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Description

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

  • Incorporates modified nucleotides(e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
  • Active in multiple buffers, including restriction enzyme, PCR, and RT buffers

Applications

  • DNA labeling by nick-translation in conjunction with DNase
  • Second-strand synthesis of cDNA in conjunction with RNase

Specifications

Concentration 10 U/μL
Description DNA Polymerase I
Polymerase DNA Polymerase I
Quantity 500 units

Frequently Asked Questions (FAQs)

What are the differences between Thermo Scientific DNA Polymerase I, Klenow Fragment, and Klenow Fragment (exo-)?

Thermo Scientific DNA Polymerase I possesses 5′?3′ DNA synthesis activity, 3′?5′ exonuclease (proofreading) activity, and 5′?3′ exonuclease activity. Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity and 3′?5′ exonuclease (proofreading) activity, but lacks 5′?3′ exonuclease activity. Klenow Fragment, exo-, is also the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity, but lacks the 3′?5′ and 5′?3′ exonuclease activities.

For Research Use Only. Not for use in diagnostic procedures.