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Applied Biosystems™ 3500 Dx Genetic Analyzer CS2 Install Kit

Catalog No. 4462042
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1000 Reactions Kit
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4462042 1000 Reactions Kit
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Catalog No. 4462042 Supplier Applied Biosystems™ Supplier No. 4462042

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

The Data Collection software utilizes the multicomponent matrix to automatically correct for the spectral overlap in samples labeled with DS-02 dyes.

The Data Collection software utilizes the multicomponent matrix to automatically correct for the spectral overlap in samples labeled with DS-02 dyes.

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1 Kit

Specifications

Format Kit
For Use With (Equipment) 3500 Dx Genetic Analyzer
For Use With (Application) Sequencing
Product Type 3500 Dx Genetic Analyzer CS2 Install Kit
Quantity 1000 Reactions Kit
When I started my run on the Applied Biosystems 3500/3500xL Genetic Analyzer, an error occurred: "Unstable Electrophoresis current detected, check for air bubbles" but I don't see any bubbles. What else can cause this?

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Running Buffer that was:
-On the instrument longer than 2 weeks
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.

Do I need to replace the buffer every 14 days if the Applied Biosystems 3500/3500xL Genetic Analyzer is not in use?

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer containers prior to starting a new run.

For research use only