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Invitrogen™ Countess™ Cell Counting Chamber Slides
Description
The Countess Chamber Slides are designed for use with the Countess 3 and 3FL automated cell counters.
The disposable Countess Cell Counting Chamber Slides offer convenience in automated cell counting for processing multiple cell samples, busy cell culture facilities, and easy disposal of contaminated or hazardous samples. They also eliminate the risk of contamination from residual samples. After use, Countess slides can be disposed of as standard biohazard waste.
The Countess Reusable Slide is a green and cost-efficient alternative to disposable slides for automated cell counting. The reusable slide enables researchers and cell culture facilities to reduce costs and generate less waste per year, making it affordable for labs to automate their cell counts. The reusable slide is cleaned and dried between counts, just as with a hemocytometer.
Each Countess Cell Counting Chamber Slide has two separate, enclosed chambers for counting one sample in duplicate or two different samples. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 μL, the same as counting four 1-mm2 squares in a standard hemocytometer.
The Countess Automated Cell Counter works in three simple steps:
- Mix 10 μL of sample with 10 μL of a cell viability stain or other desired reagent, including ReadyCount™ stains, and pipet into a Countess chamber slide
- Insert the slide into the instrument
- Press 'Count'
That's it—get the total cell count, percent viable cells, and average cell size in as little as 30 seconds.
Specifications
Specifications
| Type | Cell Counting Chamber Slide |
| Product Line | Countess |
| For Use With (Equipment) | Countess Automated Cell Counter |
| Holds | 10 μL/Chamber |
| Includes | Trypan Blue Stain, 2 x 1 mL Vials |
| Positions | 2 Chambers/Slide |
| Depth (Metric) | 100 μm (Chamber) |
| Dimensions | 75 x 25 x 1.8 mm (Chamber) |
| Content And Storage | • Countess™ Cell Counting Chamber Slides, 1 x 50 slides • Trypan blue stain, 2 x 1 mL vials Each slide contains two cell counting chambers. For use at room temperature under normal laboratory conditions. |
| Quantity | 50 Slides |
Frequently Asked Questions (FAQs)
Yes. The alternative options for Countess Cell Counting Chamber Slides (Cat. No. C10283) and for Countess 3 and Countess 3 FL Automated Cell Counter can be found using the link below:
Countess Cell Counting Chamber Slides (Cat. Nos. C10228, C10312, C10313, C10314, C10315)
The Countess Cell Counting Chamber Slides (Cat. No. C10228, C10312, C10314, C10315) are compatible with all Countess Automated Cell Counter instruments.
The Countess II and Countess II FL instruments come precalibrated. You do not have to calibrate the instruments.
It is based on dye exclusion using 0.2% Trypan Blue dye. Trypan Blue is excluded by the live cells and enters the dead cells; this results in intense staining of the intracellular proteins.
The following cell lines and primary cells have been tested:
- Cell lines: HEK 293, A431, CHO-M1, CHSE, COLO-205, COS-7, HeLa, HepG2, HL-60, J774(MMM), Jurkat, K-562, MCF-7, MRC-5, NIH/3T3, PC-12, SF-21, U266, and U2OS
- Primary cells: Human adipose tissue-derived stem cells, HASMCs, HPAECs, HPASMCs, HUVECs, C2C12, RBCs, and PMBCs
We are continually testing additional cell types.
For Research Use Only. Not for use in diagnostic procedures.