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Invitrogen™ Countess™ Cell Counting Chamber Slides

Catalog No. C10228
Encompass
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Type:
Cell Counting Chamber Slide
Reusable Slide
Includes:
No Stain
SafeCount Stain, 2 x 1 mL Vials
Trypan Blue Stain, 100 mL Bottle
Trypan Blue Stain, 2 x 1 mL Vials
Trypan Blue Stain, 2 x 100 mL Bottles
Trypan Blue Stain, 20 x 1 mL Vials
Quantity:
1 Glass Slide
1250 Slides
2500 Slides
50 Slides
500 Slides
5000 Slides
8 product options available for selection
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Catalog No. Type Includes Quantity
C10228 Cell Counting Chamber Slide Trypan Blue Stain, 2 x 1 mL Vials 50 Slides
A25750 Reusable Slide No Stain 1 Glass Slide
A40008469 Cell Counting Chamber Slide SafeCount Stain, 2 x 1 mL Vials 50 Slides
C10283 Cell Counting Chamber Slide No Stain 50 Slides
C10312 Cell Counting Chamber Slide Trypan Blue Stain, 20 x 1 mL Vials 500 Slides
C10313 Cell Counting Chamber Slide Trypan Blue Stain, 100 mL Bottle 1250 Slides
C10314 Cell Counting Chamber Slide Trypan Blue Stain, 100 mL Bottle 2500 Slides
C10315 Cell Counting Chamber Slide Trypan Blue Stain, 2 x 100 mL Bottles 5000 Slides
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Catalog No. C10228 Supplier Invitrogen™ Supplier No. C10228

Countess Cell Counting Chamber Slides are conveniently packaged for use with the Countess Automated Cell Counters. Each disposable and reusable slide features two enclosed chambers.

The Countess Chamber Slides are designed for use with the Countess 3 and 3FL automated cell counters.

The disposable Countess Cell Counting Chamber Slides offer convenience in automated cell counting for processing multiple cell samples, busy cell culture facilities, and easy disposal of contaminated or hazardous samples. They also eliminate the risk of contamination from residual samples. After use, Countess slides can be disposed of as standard biohazard waste.

The Countess Reusable Slide is a green and cost-efficient alternative to disposable slides for automated cell counting. The reusable slide enables researchers and cell culture facilities to reduce costs and generate less waste per year, making it affordable for labs to automate their cell counts. The reusable slide is cleaned and dried between counts, just as with a hemocytometer.

Each Countess Cell Counting Chamber Slide has two separate, enclosed chambers for counting one sample in duplicate or two different samples. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 μL, the same as counting four 1-mm2 squares in a standard hemocytometer.

The Countess Automated Cell Counter works in three simple steps:

  • Mix 10 μL of sample with 10 μL of a cell viability stain or other desired reagent, including ReadyCount™ stains, and pipet into a Countess chamber slide
  • Insert the slide into the instrument
  • Press 'Count'

That's it—get the total cell count, percent viable cells, and average cell size in as little as 30 seconds.

Specifications

Type Cell Counting Chamber Slide
Product Line Countess
For Use With (Equipment) Countess Automated Cell Counter
Holds 10 μL/Chamber
Includes Trypan Blue Stain, 2 x 1 mL Vials
Positions 2 Chambers/Slide
Depth (Metric) 100 μm (Chamber)
Dimensions 75 x 25 x 1.8 mm (Chamber)
Content And Storage • Countess™ Cell Counting Chamber Slides, 1 x 50 slides
• Trypan blue stain, 2 x 1 mL vials

Each slide contains two cell counting chambers.
For use at room temperature under normal laboratory conditions.
Quantity 50 Slides
Do you offer an alternative for Countess Cell Counting Chamber Slides (Cat. no. C10283) for Countess 3 and Countess 3 FL Automated Cell Counter?

Yes. The alternative options for Countess Cell Counting Chamber Slides (Cat. No. C10283) and for Countess 3 and Countess 3 FL Automated Cell Counter can be found using the link below:

Countess Cell Counting Chamber Slides (Cat. Nos. C10228, C10312, C10313, C10314, C10315)

What Countess Automated Cell Counter instruments are compatible with the Countess Cell Counting Chamber Slides (Cat. No. C10228, C10312, C10314, C10315)?

The Countess Cell Counting Chamber Slides (Cat. No. C10228, C10312, C10314, C10315) are compatible with all Countess Automated Cell Counter instruments.

How often do I have to calibrate the Invitrogen Countess II and Invitrogen Countess II FL instruments?

The Countess II and Countess II FL instruments come precalibrated. You do not have to calibrate the instruments.

How do the Invitrogen Countess II and InvitrogenCountess II FL instruments distinguish live and dead cells?

It is based on dye exclusion using 0.2% Trypan Blue dye. Trypan Blue is excluded by the live cells and enters the dead cells; this results in intense staining of the intracellular proteins.

What cell types have been tested with the Invitrogen Countess II and Invitrogen Countess II FL instruments?

The following cell lines and primary cells have been tested:

- Cell lines: HEK 293, A431, CHO-M1, CHSE, COLO-205, COS-7, HeLa, HepG2, HL-60, J774(MMM), Jurkat, K-562, MCF-7, MRC-5, NIH/3T3, PC-12, SF-21, U266, and U2OS

- Primary cells: Human adipose tissue-derived stem cells, HASMCs, HPAECs, HPASMCs, HUVECs, C2C12, RBCs, and PMBCs

We are continually testing additional cell types.

What count-to-count precision should be expected with the Invitrogen Countess II and Invitrogen Countess II FL instruments?

When an experienced worker manually counts cells using a glass hemocytometer together with a microscope, count-to-count variability of a single sample is commonly 10% or more. Users of Countess II and Countess II FL instruments should typically see <5% count-to-count variability.

With the Invitrogen Countess II and Invitrogen Countess II FL instruments, why do my brightfield and fluorescent counts differ by 2x?

When counting in brightfield mode, the Countess II and Countess II FL instruments automatically assume a 1:1 dilution has been made with trypan blue. The same assumption is not made when in fluorescence mode.

What is the counting range with Invitrogen Countess II instruments?

The Countess II instruments are designed to read samples with concentrations in the range of 1 x 10E4 cells/mL to 1 x 10E7 cells/mL.

How long do the Invitrogen Countess II and Invitrogen Countess II FL instruments take to count cells?

The time taken is ˜10 seconds per sample.

What size of cells can the Invitrogen Countess II Automated Cell Counter and Invitrogen Countess II FL Automated Cell Counter count?

The Countess II and Countess II FL instruments can detect particles/cells ranging from 4 - 60 µm. Cells ranging from 7 - 60 µm can be counted and assessed for viability with brightfield viewing.


For Research Use Only. Not for use in diagnostic procedures.