Clone: MID 15B4. Host: Mouse. Isotype: IgM. Immunogen: Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]) conjugated to BSA. Source: Purified from ascites by gel filtration on sephacryl S-300. Species Reactivity: Species independent. Specificity: Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly artificial lipid overlay test systems. Under more physiological in vitro and in vivo conditions highly specific for ceramide and does not cross-react with sphingomyelin, cholesterol or other phospholipids. Applications: ELISA. Flow Cytometry, ICC, IHC (PS). Recommended dilutions: ELISA (1:10). Immunohistochemistry (1:10). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Formulation: Liquid. In PBS, pH 7.2, containing 0.5M sodium chloride, 0.1% BSA and 0.09% sodium azide.
