Abnova™ CDKN2A/CEN9 CISH Probe

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (CDKN2A gene), and dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 9).
Normal situation: In interphases of normal cells or cells without a aberrations involving the 9p21.3 band, two green and two red signals per nuclei appear.
Aberrant situation: In a cell with deletions affecting the CDKN2A locus, a reduced number of green signals will be observed. Deletions affecting only parts of the CDKN2A gene might result in a normal signal pattern with green signals of reduced size.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.