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  6. Invitrogen™ CD45.2 Monoclonal Antibody (104), NovaFluor™ Yellow 810, eBioscience™

Invitrogen™ CD45.2 Monoclonal Antibody (104), NovaFluor™ Yellow 810, eBioscience™

Numéro de catalogue. M027T03Y09A
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100 μg
25 μg
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Numéro de catalogue. Quantité
M027T03Y09A 100 μg
M027T02Y09A 25 μg
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Numéro de catalogue. M027T03Y09A Fournisseur Invitrogen™ Code fournisseur M027T03Y09A

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Description

Mouse Monoclonal Antibody

The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.Applications TestedThe 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Master Mixes

  • Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time.
  • We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer.Whole Blood Compatibility
  • When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.
  • See the Bulk Lysis of Human Whole Blood protocol here.
  • Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-...

    CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

    • Spécifications

    Antigène CD45.2
    Applications Flow Cytometry
    Classification Monoclonal
    Clone 104
    Concentration 0.1 mg/mL
    Conjugué NovaFluor Yellow 810
    Formule PBS with BSA and 0.09% sodium azide; pH 7.2
    Gène Ptprc
    Numéro d’acquisition du gène P06800
    Alias du gène B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
    Symboles de gène Ptprc
    Espèces hôtes Mouse
    Méthode de purification Affinity chromatography
    Quantité 100 μg
    Statut réglementaire RUO
    Primaire ou secondaire Primary
    ID du gène (Entrez) 19264
    Espèces cibles Mouse
    Contenu et stockage 4°C, store in dark, DO NOT FREEZE!
    Type de produit Antibody
    Forme Liquid
    Isotype IgG2a κ
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