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  6. Invitrogen™ CD15 Monoclonal Antibody (HI98), PE, eBioscience™

Invitrogen™ CD15 Monoclonal Antibody (HI98), PE, eBioscience™
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Numéro de catalogue. 501122214
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100 Tests
25 Tests
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Numéro de catalogue. Quantité
501122214 100 Tests
12015941 25 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
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Numéro de catalogue. 501122214 Fournisseur Invitrogen™ Code fournisseur 12015942

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Description

Mouse Monoclonal Antibody

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative. Applications Reported: This HI98 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Spécifications

Antigène CD15
Applications Flow Cytometry
Classification Monoclonal
Clone HI98
Concentration 5 μL/Test
Conjugué PE
Formule PBS with BSA and 0.09% sodium azide; pH 7.2
Gène FUT4
Numéro d’acquisition du gène P22083
Alias du gène FUTIV; Lewis X; SSEA-1
Symboles de gène FUT4
Espèces hôtes Mouse
Méthode de purification Affinity chromatography
Quantité 100 Tests
Statut réglementaire RUO
Primaire ou secondaire Primary
ID du gène (Entrez) 2526
Espèces cibles Human
Contenu et stockage 4°C, store in dark, DO NOT FREEZE!
Type de produit Antibody
Forme Liquid
Isotype IgM κ
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