Invitrogen™ CD11c Monoclonal Antibody (3.9), Brilliant Ultra Violet™ 661, eBioscience™

Mouse Monoclonal Antibody

The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.Applications TestedThis 3.9 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (1.0 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Blocking BuffersWhen using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivityThis tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation- Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 ...